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cc-by-nc-nd (c) Elsevier Ltd, 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/200821

Impact of fertilization with pig slurry on the isotopic composition of nitrate retained in soil and leached to groundwater in agricultural areas

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The isotopic composition of N and O of nitrate (NO3−) is usually employed to trace its sources of pollution in groundwater. In agricultural areas, the amount of NO3− that reaches the aquifers after fertilization is controlled by different transformation processes that can affect the nitrogen species isotopic composition. Aiming to address the reliability of using isotope tools to trace sources of groundwater NO3−, the goal of this study was to check the effect of fertilization on the isotopic composition of N compounds retained and leached from soils. The concentration and isotopic composition (δ15N and δ18O) of ammonium (NH4+), NO3− and nitrite (NO2−) was characterized after the application of pig slurry in lysimeters containing either soil under fallow (LF) or the same soil continuously cropped and fertilized (LC) during the previous six years. Results showed that the leached NO3− isotopic signature did not directly reflect the isotopic composition of the applied pig slurry. Just after fertilization, nitrification led to lower δ15NNO3 values in soil extracts and leachates (e.g. from +5.9 ± 0.9¿ to +3.8 ± 3.1¿ in soil extracts of LF lysimeters). These values increased after complete nitrification (+11.5 ± 1.3¿) towards the δ15Nbulk of pig slurry (+19.6 ± 0.5¿). Later on, due to soil organic matter and plant debris mineralization and subsequent nitrification, values decreased towards the initial δ15NNO3 of soil but remained above them (+8.6 ± 1.0¿). Both LF and LC experiments showed a similar trend and the latter ones allowed to reinforce that long-term fertilization with pig slurry can increase the soil δ15NNO3. Concerning the δ18O of NO3− from soil extracts and leachates, it mainly depended on the δ18O of irrigation water and oxygen, after nitrification of NH4+ from pig slurry. Therefore, studies aiming to trace groundwater NO3− pollution sources in rural areas by using an isotopic approach should consider the fertilization history of each setting. Also, analyzing the δ15Nbulk of soil is recommended, since it could mask the isotopic signature of the N applied through fertilization.

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MARGALEF MARTI, Rosanna, et al. Impact of fertilization with pig slurry on the isotopic composition of nitrate retained in soil and leached to groundwater in agricultural areas. Applied Geochemistry. 2021. Vol. 125, num. 104832. ISSN 0883-2927. [consulted: 21 of May of 2026]. Available at: https://hdl.handle.net/2445/200821

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