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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/125936
PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
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We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel of colorectal and pancreatic cell lines in CM from CAFlm resulted in overexpression of mRNA PRL-3 and higher overexpression in CAFs than in non-activated fibroblasts. Moreover PRL-3 mRNA expression correlates with expression of alpha-SMA and deposition of collagen fibrils in the stroma. We demonstrate that products secreted by CAFs trigger PRL-3 overexpression in cancer cells. Identification of these factors may contribute to new stroma-targeted therapies for desmoplastic tumours
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GARCIA I MOLLEVÍ, David, AYTÉS MENESES, Álvaro, BERDIEL ACER, Mireia, PADULLÉS MOSELLA, Laura, MARTÍNEZ INIESTA, María, SANJUAN, Xavier, SALAZAR SOLER, Ramón, VILLANUEVA GARATACHEA, Alberto. PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts. _Molecular Cancer_. 2009. Vol. 8, núm. 46. [consulta: 23 de gener de 2026]. ISSN: 1476-4598. [Disponible a: https://hdl.handle.net/2445/125936]