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cc by (c) Zboromyrska, Yuliya et al, 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/197926

Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis

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The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.

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ZBOROMYRSKA, Yuliya, RICO, Verónica, PITART, Cristina, FERNANDEZ PITTOL, Mariana josé, SORIANO, Àlex, BOSCH MESTRES, Jordi. Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis. _Antibiotics_. 2022. Vol. 11, núm. 5. [consulta: 3 de febrer de 2026]. ISSN: 2079-6382. [Disponible a: https://hdl.handle.net/2445/197926]

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