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Implementation of a New Protocol for Direct Identification_Antibiotics.pdf (429.7 KB)

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2022-04-26

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cc by (c) Zboromyrska, Yuliya et al, 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/197926

Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis

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https://doi.org/10.3390/antibiotics11050582

Abstract

The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.

Subject

Infeccions del tracte urinari, Bacteris patògens

Subject (English)

Urinary tract infections, Pathogenic bacteria

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Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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ZBOROMYRSKA, Yuliya, et al. Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis. Antibiotics. 2022. Vol. 11, num. 5. ISSN 2079-6382. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/197926

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