Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis

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dc.contributor.authorZboromyrska, Yuliya
dc.contributor.authorRico, Verónica
dc.contributor.authorPitart, Cristina
dc.contributor.authorFernandez Pittol, Mariana José
dc.contributor.authorSoriano,Àlex
dc.contributor.authorBosch Mestres, Jordi
dc.date.accessioned2023-05-12T11:31:38Z
dc.date.available2023-05-12T11:31:38Z
dc.date.issued2022-04-26
dc.date.updated2023-05-02T07:24:46Z
dc.description.abstractThe direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.
dc.format.extent6 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idimarina9315280
dc.identifier.issn2079-6382
dc.identifier.pmid35625226
dc.identifier.urihttps://hdl.handle.net/2445/197926
dc.language.isoeng
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/antibiotics11050582
dc.relation.ispartofAntibiotics, 2022, vol. 11, num. 5
dc.relation.urihttps://doi.org/10.3390/antibiotics11050582
dc.rightscc by (c) Zboromyrska, Yuliya et al, 2022
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.sourceArticles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
dc.subject.classificationInfeccions del tracte urinari
dc.subject.classificationBacteris patògens
dc.subject.otherUrinary tract infections
dc.subject.otherPathogenic bacteria
dc.titleImplementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)